SubtiBank SubtiBank
Version comparison:

2018-09-20 18:43:532025-05-24 07:03:36

description

similar to serine O-acetyltransferase, extracellular polysaccharide synthesis

UDP-2,4,6-trideoxy-2-acetamido-4-amino glucose acetyltransferase, extracellular polysaccharide synthesis

locus

BSU34240

BSU_34240

geneLength

648

651

product

unknown

UDP-2,4,6-trideoxy-2-acetamido-4-amino glucose acetyltransferase

outlinks

bsu

BSU34240

BSU_34240

Gene

Coordinates

3,516,233 → 3,516,883

3,516,233 3,516,883

The protein

Protein family

transferase hexapeptide repeat family (according to Swiss-Prot)

[SW|Transferase hexapeptide repeat family] (according to UniProt)

Biological materials

Mutant

MGNA-B609 (yvfD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1608 NBRP B. subtilis, Japan]

BKE34240 (Δ[[gene|epsM]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCACCCACAATGGCCACAT, downstream forward: _UP4_CAAACATCAAACAAAGGATG

BKK34240 (Δ[[gene|epsM]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCACCCACAATGGCCACAT, downstream forward: _UP4_CAAACATCAAACAAAGGATG

MGNA-B609 (yvfD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1608 NBRP B. subtilis, Japan]

BKE34240 ([[gene|epsM]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCACCCACAATGGCCACAT, downstream forward: _UP4_CAAACATCAAACAAAGGATG

BKK34240 ([[gene|epsM]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCACCCACAATGGCCACAT, downstream forward: _UP4_CAAACATCAAACAAAGGATG

Labs working on this gene/protein

[SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

References

Research papers

30222950

30222950, 30314705, 24064219

=The EAR [SW|RNA switch]=

20374491, 20230605

The protein

Catalyzed reaction/ biological activity

conversion of UDP-2,4,6-trideoxy-2-acetamido-4-amino glucose to UDP-2,4,6-trideoxy-2,4-diacetamido glucose (N,N'-diacetylbacillosamine) [pubmed|30314705]

The protein

[SW|Cofactors]

coenzyme A [pubmed|30314705]

The protein

Structure

[PDB|4M99] (from Neisseria gonorrhoeae, 38% identity) [pubmed|24064219]

labs

[SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

References

The EAR [SW|RNA switch]

20374491, 20230605